product summary
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company name :
HUABIO
product type :
antibody
product name :
Histone H3 (mono methyl K18)
catalog :
ET1601-14
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
SA42-07
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Histone H3 (mono methyl K18) on HepG2 cell lysates with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Histone H3 (mono methyl K18) on mouse testis tissue lysates with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-14) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 3 :
ICC staining Histone H3 (mono methyl K18) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ET1601-14
Target name :
Histone H3 (mono methyl K18)
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IF-Tissue,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Histone H3 aa 1-50 / 136 (mono methyl K18) conjugated to Keyhole Limpet Haemocyanin (KLH).
Uniprot id :
P68431>SwissProt: P68431 Human;SwissProt: P84243 Human;SwissProt: Q71DI3 Human;SwissProt: Q16695 Human;SwissProt: Q6NXT2 Human;SwissProt: P68433 Mouse;SwissProt: Q6LED0 Rat
Host :
Rabbit
Clone number :
SA42-07
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HepG2 cell lysates, mouse testis tissue lysates, HepG2, PC-3M, human colon tissue, mouse testis tissue, mouse kidney tissue.
Molecular wt :
Predicted band size: 15 kDa
Subcellular location :
Nucleus, Chromosome
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:5,000
;IF-Cell: 1:50
;IF-Tissue: 1:50
;IHC-P: 1:50-1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1601-14_4.jpg
Pic legend4 :
ICC staining Histone H3 (mono methyl K18) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1601-14_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Histone H3 (mono methyl K18) antibody (ET1601-14) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-14) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1601-14_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1601-14_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1601-14_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Histone H3 (mono methyl K18) antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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