LRP1 | HUABIO ET1601-1-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LRP1

catalog :

ET1601-1-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

SA0290

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Human lung tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of LRP1 on different lysates with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. Lane 1: Rat lung tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 505 kDa Observed band size: 80 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-1) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

All lanes: Western blot analysis of LRP1 with anti-LRP1 antibody (ET1601-1) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: LRP1 knockdown Hela whole cell lysate (10 µg). ET1601-1 was shown to specifically react with LRP1 in wild-type Hela cells. Weakened bands were observed when LRP1 knockdown samples were tested. Wild-type and LRP1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1601-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1601-1-50UL

Target name :

LRP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human LRP1 aa 4,471-4,520 / 4,544.

Uniprot id :

Q07954>SwissProt: Q07954 Human;SwissProt: Q91ZX7 Mouse;SwissProt: G3V928 Rat

Host :

Rabbit

Clone number :

SA0290

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse liver tissue lysate, Human lung tissue lysate, Rat liver tissue lysate, Rat lung tissue lysate, Mouse lung tissue lysate, Human liver tissue lysate, mouse brain tissue, rat brain tissue, rat liver tissue, human lung tissue, human liver tissue, mouse liver tissue.

Molecular wt :

Predicted band size: 505 kDa

Subcellular location :

Cytoplasm, Nucleus, Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Tissue: 1:50 ;IHC-P: 1:200-1:2,000 ;IP: 1-2μg/sample ;IHC-Fr: 1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-LRP1 antibody (ET1601-1) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1601-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_10.jpg

Pic legend10 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_11.jpg

Pic legend11 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling LRP1 with Rabbit anti-LRP1 antibody (ET1601-1). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1601-1, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1601-1_12.jpg

Pic legend12 :

LRP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1601-1 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1601-1 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1601-1 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1601-1 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

more info or order :

HUABIO product webpage