BMP2 | HUABIO ER80602 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BMP2

catalog :

ER80602

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (ER80602) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: C2C12 cell lysate Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER80602) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining BMP2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

image 3 :

ICC staining BMP2 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).

product information

SKU :

ER80602

Target name :

BMP2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human BMP2 aa 51-100.

Uniprot id :

P12643>SwissProt: P12643 Human;SwissProt: P21274 Mouse;SwissProt: P49001 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Caco-2 cell lysate, C2C12 cell lysate, Hela, MCF-7, rat small intestine tissue, human breast cancer tissue, mouse small intestine tissue.

Molecular wt :

Predicted band size: 45 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER80602_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER80602_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER80602_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-BMP2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER80602_7.jpg

Pic legend7 :

Flow cytometric analysis of Hela cells with BMP2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage