SOCS3 | HUABIO ER65765 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

SOCS3

catalog :

ER65765

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SOCS3 on different lysates with Rabbit anti-SOCS3 antibody (ER65765) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: Mouse spleen tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Rat spleen tissue lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 25 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65765) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOCS3 antibody (ER65765) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65765) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-SOCS3 antibody (ER65765) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER65765) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER65765

Target name :

SOCS3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human

Uniprot id :

O14543>SwissProt: O14543 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

PC-3M cell lysate, mouse spleen tissue lysate, mouse colon tissue lysate, rat spleen tissue lysate, RAW264.7 cell lysate, human colon tissue, mouse spleen tissue, HeLa.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cytoplasmic side of plasma membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:500 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER65765_4.jpg

Pic legend4 :

Flow cytometric analysis of HeLa cells labeling SOCS3. Cells were fixed and permeabilized. Then stained with the primary antibody (ER65765, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage