product summary
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company name :
HUABIO
product type :
antibody
product name :
DAAM1
catalog :
ER65757
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of DAAM1 on different lysates with Rabbit anti-DAAM1 antibody (ER65757) at 1/20,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65757) at 1/20,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of DAAM1 on Neuro-2a cell lysates with Rabbit anti-DAAM1 antibody (ER65757) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65757) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ER65757
Target name :
DAAM1
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human DAAM1 aa 1-150.
Uniprot id :
Q9Y4D1>SwissProt: Q9Y4D1 Human;SwissProt: Q8BPM0 Mouse;Entrez Gene: 314212 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
HepG2 cell lysate, HCT 116 cell lysate, MDA-MB-231 cell lysate, PC-12 cell lysate, Neuro-2a cell lysates.
Molecular wt :
Predicted band size: 123 kDa
Subcellular location :
Cytoplasm, cytoskeleton, cilium basal body.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:20,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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