product summary
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company name :
HUABIO
product type :
antibody
product name :
ULK3
catalog :
ER65265
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (ER65265) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ULK3 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65265) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (ER65265) at 1/2,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65265) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Flow cytometric analysis of HeLa cells labeling ULK3.
Cells were fixed and permeabilized. Then stained with the primary antibody (ER65265, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ER65265
Target name :
ULK3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ULK3 aa 1-472 / 472.
Uniprot id :
Q6PHR2>SwissProt: Q6PHR2 Human;SwissProt: Q3U3Q1 Mouse;SwissProt: D3ZHP7 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
293T cell lysate, HeLa cell lysate, Jurkat cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa.
Molecular wt :
Predicted band size: 53 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;FC: 1:1,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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