E-Cadherin | HUABIO ER63312 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

E-Cadherin

catalog :

ER63312

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Colon Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Colon Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

image 3 :

Western blot analysis of E-Cadherin on different lysates with Rabbit anti-E-Cadherin antibody (ER63312) at 1/10,000 dilution. Lane 1: T-47D cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (negative) (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: 4T1 cell lysate (20 µg/Lane) Lane 5: C2C12 cell lysate (negative) (20 µg/Lane) Lane 6: Mouse pancreas tissue lysate (20 µg/Lane) Lane 7: Rat pancreas tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 80-130 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63312) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ER63312

Target name :

E-Cadherin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,IHC-Fr,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse E-Cadherin aa 151-730.

Uniprot id :

P12830>SwissProt: P12830 Human;SwissProt: P09803 Mouse;SwissProt: Q9R0T4 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

T-47D cell lysate, HCT 116 cell lysate, 4T1 cell lysate, Mouse pancreas tissue lysate, Rat pancreas tissue lysate, MCF7, 4T1, mouse pancreas tissue, rat pancreas tissue, mouse colon tissue, rat colon tissue.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Cell junction, adherens junction, Cell membrane, Endosome, Golgi apparatus, trans-Golgi network, Cytoplasm, desmosome.

Concentration :

2 mg/mL.

Recommended dilutions :

WB: 1:10,000 ;IHC-P: 1:2,000 ;IF-Cell: 1:500 ;IHC-Fr: 1:1,000 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER63312_4.jpg

Pic legend4 :

Immunocytochemistry analysis of MCF7 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (ER63312) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (ER63312) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER63312_5.jpg

Pic legend5 :

Immunocytochemistry analysis of 4T1 cells labeling E-Cadherin with Rabbit anti-E-Cadherin antibody (ER63312) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-E-Cadherin antibody (ER63312) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. C2C12 is a negative control cell.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER63312_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-E-Cadherin antibody (ER63312) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63312) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER63312_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-E-Cadherin antibody (ER63312) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63312) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER63312_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-E-Cadherin antibody (ER63312) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63312) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER63312_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-E-Cadherin antibody (ER63312) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER63312) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ER63312_10.jpg

Pic legend10 :

Flow cytometric analysis of MCF7 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER63312, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ER63312_11.jpg

Pic legend11 :

Flow cytometric analysis of 4T1 cells labeling E-Cadherin. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER63312, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage