VEGF Receptor 1 | HUABIO ER50803 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

VEGF Receptor 1

catalog :

ER50803

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of VEGF Receptor 1 on different lysates with Rabbit anti-VEGF Receptor 1 antibody (ER50803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER50803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of VEGFR1 on different lysates with Rabbit anti-VEGFR1 antibody (ER50803) at 1/1,000 dilution. Lane 1: MCF7-si NT cell lysate Lane 2: MCF7-si VEGFR1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 151 kDa Observed band size: 151 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. ER50803 was shown to specifically react with VEGFR1 in Hela-si NT cells. Weakened band was observed when Hela-si VEGFR1 sample was tested. Hela-si NT and Hela-si VEGFR1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER50803, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER50803

Target name :

VEGF Receptor 1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human VEGFR1.

Uniprot id :

P17948>SwissProt: P17948 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse liver tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, human liver tissue, human kidney tissue.

Molecular wt :

Predicted band size: 151 kDa

Subcellular location :

Cell membrane, endosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER50803_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER50803_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER50803_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VEGFR1 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage