Chromogranin A | HUABIO ER40724 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Chromogranin A

catalog :

ER40724

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2,000 dilution.

image 2 :

Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER40724, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

image 3 :

Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER40724, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

ER40724

Target name :

Chromogranin A

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,IF-Tissue,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to C-terminal of Mouse Chromogranin A aa 408-457 / 457 (ID: P10645).

Uniprot id :

P10645>SwissProt: P10645 Human;SwissProt: P26339 Mouse;SwissProt: P10354 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

PC12 cell lysate, human pancreas tissue, mouse pancreas tissue, rat pancreas tissue, human colon cancer tissue, Lovo.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Cytoplasmic vesicle, secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:100-1:200 ;IF-Tissue: 1:200 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER40724_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Chromogranin A antibody (ER40724) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40724) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER40724_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER40724_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER40724_7.jpg

Pic legend7 :

Flow cytometric analysis of Lovo cells with chromogranin A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER40724_8.jpg

Pic legend8 :

Western blot analysis of Chromogranin A on different lysates with Rabbit anti-Chromogranin A antibody (ER40724) at 1/1,000 dilution. Lane 1: PC-12 cell lysate Lane 1: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50-75 kDa Observed band size: 50-75 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40724) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER40724_9.jpg

Pic legend9 :

Immunocytochemistry analysis of PC-12 cells labeling Chromogranin A with Rabbit anti-Chromogranin A antibody (ER40724) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Chromogranin A antibody (ER40724) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage