Topoisomerase II alpha | HUABIO ER40124 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Topoisomerase II alpha

catalog :

ER40124

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TOP2A on different lysates using anti-TOP2A antibody at 1/500 dilution. Positive control: Lane 1: Jurkat cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: A431 cell lysate Lane 4: L929 cell lysate Lane 5: A549 cell lysate Lane 6: Human liver tissue lysate Lane 7: HUVEC cell lysate

image 2 :

ICC staining TOP2A in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining TOP2A in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER40124

Target name :

Topoisomerase II alpha

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal residues of Topoisomerase II alpha.

Uniprot id :

P11388>SwissProt: P11388 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, NIH/3T3 cell lysate, A431 cell lysate, L929 cell lysate, A549 cell lysate, Human liver tissue lysate, HUVEC cell lysate, A549, Hela, A431, NIH/3T3, human tonsil tissue, human colon cancer tissue, human colon cancer tissue, Hela.

Molecular wt :

Predicted band size: 174 kDa

Subcellular location :

Cytoplasm, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER40124_4.jpg

Pic legend4 :

ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER40124_5.jpg

Pic legend5 :

ICC staining TOP2A in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER40124_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TOP2A antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER40124_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER40124_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TOP2A antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER40124_9.jpg

Pic legend9 :

Flow cytometric analysis of Hela cells with TOP2A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage