STAT6 | HUABIO ER40121 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

STAT6

catalog :

ER40121

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of STAT6 on different cell lysates using anti-STAT6 antibody at 1/1,000 dilution. Positive control: Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lane 3: Hela cell lysate Lane 4: Ags cell lysate Lane 5: Jurkat cell lysate

image 2 :

Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ER40121) at 1/500 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si STAT6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 94 kDa Observed band size: 94 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. ER40121 was shown to specifically react with STAT6 in Hela-si NT cells. Weakened band was observed when Hela-si STAT6 sample was tested. Hela-si NT and Hela-si STAT6 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER40121, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining STAT6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER40121

Target name :

STAT6

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within STAT6 aa 814-847/847.

Uniprot id :

P42226>SwissProt: P42226 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, Raji cell lysate, Daudi cell lysate, Hela cell lysate, Ags cell lysate, human tosnsil tissue, human kidney tissue.

Molecular wt :

Predicted band size: 94 kDa

Subcellular location :

Cytoplasm, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER40121_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT6 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER40121_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage