MGMT | HUABIO ER40104 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MGMT

catalog :

ER40104

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MGMT on different lysates with Rabbit anti-MGMT antibody (ER40104) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-MGMT KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40104) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MGMT on different lysates using anti- MGMT antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: MCF-7 cell lysate Lane 3: Daudi cell lysate Lane 4: Raji cell lysate Lane 5: SK-BR-3 cell lysate Lane 6: SKOV3 cell lysate Lane 7: Human liver tissue lysate Lane 8: Human kidney tissue lysate Lane 9: Human thymus tissue lysate Lane 10: Human placentae tissue lysate

image 3 :

ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER40104

Target name :

MGMT

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within residues of MGMT aa 158-207 / 207.

Uniprot id :

P16455>SwissProt: P16455 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF-7 cell lysate, Hela cell lysate, Dauai cell lysate, Raji cell lysate, SK-BR-3 cell lysate, SKOV3 cell lysate, human kidney tissue lysate, human liver tissue lysate, human thymus tissue lysate, human placentae tissue lysate, A172, SK-BR-3, MCF-7, human tonsil tissue, human colon carcinoma tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC:1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER40104_4.jpg

Pic legend4 :

ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER40104_5.jpg

Pic legend5 :

ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER40104_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER40104_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage