Cdk6 | HUABIO ER40101 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cdk6

catalog :

ER40101

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cdk6 on different lysates with Rabbit anti-Cdk6 antibody (ER40101) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40101) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CDK6 on different cell lysates using anti-CDK6 antibody at 1/2,000 dilution. Positive control: Lane 1: K562 cell lysate Lane 2: COS-1 cell lysate

image 3 :

ICC staining CDK6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER40101

Target name :

Cdk6

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Cdk6 aa 267-326 / 326.

Uniprot id :

Q00534>SwissProt: Q00534 Human;SwissProt: Q64261 Mouse;Entrez Gene: 114483 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, K562 cell lysate, COS-1 cell lysate, Hela, HepG2, human lung cancer tissue, mouse thymus tissue, rat thymus tissue.

Molecular wt :

Predicted band size: 37 kDa

Subcellular location :

Cytoplasm, nucleus, Cell projection, Cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:200 ;IHC-P: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER40101_4.jpg

Pic legend4 :

ICC staining CDK6 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER40101_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER40101_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER40101_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat thymus tissue with Rabbit anti-Cdk6 antibody (ER40101) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40101) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage