CD31 | HUABIO ER31219 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD31

catalog :

ER31219

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (10 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 120-130 kDa Exposure time: Lane 1: 16 seconds; Lane 2-4: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31219) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD31 antibody (ER31219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER31219

Target name :

CD31

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal residues of CD31.

Uniprot id :

P16284>SwissProt: P16284 Human;SwissProt: Q08481 Mouse;SwissProt: Q3SWT0 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

THP-1 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, human lung tissue, human tonsil tissue, rat lung tissue, Hela, HUVEC, NIH/3T3, SW480, Jurkat.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Cell membrane, Cell junction.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;FC: 1:100-1:200 ;IHC-P: 1:1,000-1:5,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER31219_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-CD31 antibody (ER31219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER31219_5.jpg

Pic legend5 :

ICC staining CD31 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER31219_6.jpg

Pic legend6 :

ICC staining CD31 in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER31219_7.jpg

Pic legend7 :

ICC staining CD31 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER31219_8.jpg

Pic legend8 :

ICC staining CD31 in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER31219_9.jpg

Pic legend9 :

Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage