CDk1 | HUABIO ER31213 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CDk1

catalog :

ER31213

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of HeLa cells labeling CDk1 with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 2 :

Immunocytochemistry analysis of NIH/3T3 cells labeling CDk1 with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CDk1 antibody (ER31213) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Western blot analysis of CDk1 on different lysates using anti-CDk1 antibody at 1/500 dilution. Positive control: Lane 1: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 3: PC12 cell lysate Lane 4: HepG2 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Mouse liver tissue lysate Lane 8: SKBR3 cell lysate

product information

SKU :

ER31213

Target name :

CDk1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human CDk1.

Uniprot id :

P06493>SwissProt: P06493 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

MCF-7 cell lysate, Jurkat cell lysate, PC12 cell lysate, HepG2 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, SKBR3 cell lysate, HeLa, NIH/3T3, rat spleen tissue, human tonsil tissue, human breast cancer tissue, mouse spleen tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, nucleus, Cytoskeleton, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:100 ;IHC-P: 1:200 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER31213_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER31213_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CDk1 antibody. Counter stained with hematoxylin

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER31213_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-e.mbedded human breast cancer tissue using anti-CDk1 antibody. Counter stained with hematoxylin

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER31213_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CDk1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER31213_8.jpg

Pic legend8 :

Flow cytometric analysis of HeLa cells labeling CDk1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER31213, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage