product summary
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company name :
HUABIO
product type :
antibody
product name :
MMP-1
catalog :
ER31211
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane)
Lane 3: Raji cell lysate (15 µg/Lane)
Lane 4: A431 cell lysate (15 µg/Lane)
Lane 5: HeLa cell lysate (15 µg/Lane)
Lane 6: HUVEC cell lysate (15 µg/Lane)
Lane 7: A549 cell lysate lysate (30 µg/Lane)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: Raji cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 23 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP1 antibody. Counter stained with hematoxylin.
product information
SKU :
ER31211
Target name :
MMP-1
Species reactivity :
Human,Mouse,Monkey
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human MMP1 aa 432-475.
Uniprot id :
P03956>SwissProt: P03956 Human;SwissProt: P81563 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
SK-Br-3 cell lysate, MCF7 cell lysate, Raji cell lysate, A431 cell lysate, HeLa cell lysate, HUVEC cell lysate, A549 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, human colon cancer tissue.
Molecular wt :
Predicted band size: 54 kDa
Subcellular location :
Secreted.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;IHC-P: 1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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