GRB2 | HUABIO ER31203 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GRB2

catalog :

ER31203

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GRB2 on different lysates with Rabbit anti-GRB2 antibody (ER31203) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: MCF7 cell lysate Lane 3: HepG2 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: F9 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 25/40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31203) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining GRB2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRB2 antibody. Counter stained with hematoxylin.

product information

SKU :

ER31203

Target name :

GRB2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal residues of GRB2.

Uniprot id :

P62993>SwissProt: P62993 Human;SwissProt: Q60631 Mouse;SwissProt: P62994 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

THP-1 cell lysate, MCF7 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, PC-12 cell lysate, HepG2, human tonsil tissue, human breast cancer tissue, SHG-44.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cytoplasm, nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER31203_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER31203_5.jpg

Pic legend5 :

Flow cytometric analysis of SHG-44 cells with GRB2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage