CD133 | HUABIO ER31008 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD133

catalog :

ER31008

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD133 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER31008, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SW480 cell lysate Lane 3: HCT116 cell lysate Lane 4: HT-29 cell lysate Lane 5: HepG2 cell lysate Lane 6: 293 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD133 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31008, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Flow cytometric analysis of SHG-44 cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

product information

SKU :

ER31008

Target name :

CD133

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD133 aa 179-389.

Uniprot id :

O43490>SwissProt: O43490 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, SW480 cell lysate, HCT116 cell lysate, HT-29 cell lysate, HepG2 cell lysate, 293 cell lysate, human breast tissue.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:500-1:1,000 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER31008_4.jpg

Pic legend4 :

Flow cytometric analysis of HUVEC cells with CD133 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage