Pro Caspase-3 | HUABIO ER30804 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Pro Caspase-3

catalog :

ER30804

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Pro Caspase-3 on different lysates with Rabbit anti-Pro Caspase-3 antibody (ER30804) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate Lane 3: Human brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30804) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of Caspase-3 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining of Caspase-3 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER30804

Target name :

Pro Caspase-3

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IHC-P,FC,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Caspase-3.

Uniprot id :

P42574>SwissProt: P42574 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Jurkat cell lysate, Ramos cell lysate, human brain tissue lysate, HepG2, A549, human liver carcinoma tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 32 kDa

Subcellular location :

Nucleus, cytoplasm,

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200 ;IHC-Fr:1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER30804_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER30804_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-3 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER30804_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells with Caspase-3 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER30804_7.jpg

Pic legend7 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Pro Caspase-3 with Rabbit anti-Pro Caspase-3 antibody (ER30804). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER30804, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER30804_8.jpg

Pic legend8 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Pro Caspase-3 with Rabbit anti-Pro Caspase-3 antibody (ER30804). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ER30804, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

more info or order :

HUABIO product webpage