VEGF | HUABIO ER30607 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

VEGF

catalog :

ER30607

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of VEGF on different lysates with Rabbit anti-VEGF antibody (ER30607) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: HUVEC cell lysate Lane 4: MCF7 cell lysate Lane 5: U-87 MG cell lysate Lane 6: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30607) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

All lanes: Western blot analysis of VEGF with anti-VEGF antibody (ER30607) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2/3: VEGF knockdown Hela whole cell lysate (10 µg). ER30607 was shown to specifically react with VEGF in wild-type Hela cells. Weakened bands were observed when VEGF knockdown samples were tested. Wild-type and VEGF knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER30607, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER30607

Target name :

VEGF

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human VEGFA (P15692-1).

Uniprot id :

P15692>SwissProt: P15692 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HeLa cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, MCF7 cell lysate, U-87 MG cell lysate, K-562 cell lysate, HepG2, HUVEC, human lung carcinoma tissue, Hela.

Molecular wt :

Predicted band size: 27 kDa

Subcellular location :

Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:200 ;IHC-P: 1:200 ;FC: 1:100-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER30607_4.jpg

Pic legend4 :

ICC staining VEGF in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER30607_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER30607_6.jpg

Pic legend6 :

Flow cytometric analysis of Hela cells with VEGF antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

more info or order :

HUABIO product webpage