product summary
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company name :
HUABIO
product type :
antibody
product name :
VCP
catalog :
ER30603
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, zebrafish
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of VCP on different lysates with Rabbit anti-VCP antibody (ER30603) at 1/2,000 dilution.
Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: HeLa cell lysate (10 µg/Lane)
Lane 4: Jurkat cell lysate (10 µg/Lane)
Lane 5: A431 cell lysate (10 µg/Lane)
Lane 6: L929 cell lysate (10 µg/Lane)
Lane 7: F9 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Neuro-2a cell lysate (10 µg/Lane)
Lane 10: Mouse heart tissue lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (20 µg/Lane)
Lane 12: PC-12 cell lysate (10 µg/Lane)
Lane 13: Rat liver tissue lysate (20 µg/Lane)
Lane 14: Rat kidney tissue lysate (20 µg/Lane)
Predicted band size: 89 kDa
Observed band size: 89 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30603) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of HeLa cells labeling VCP with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
image 3 :
Immunocytochemistry analysis of NIH/3T3 cells labeling VCP with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-VCP antibody (ER30603) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
product information
SKU :
ER30603
Target name :
VCP
Species reactivity :
Human,Mouse,Rat,Zebrafish
Applications :
WB,IF-Cell,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human VCP aa 757-806 / 806.
Uniprot id :
P55072>SwissProt: P55072 Human;SwissProt: Q01853 Mouse;SwissProt: P46462 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
A549 cell lysate, MCF7 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, L929 cell lysate, F9 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, mouse heart tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat liver tissue lysate, rat kidney tissue lysate, HeLa, NIH/3T3, human uterus tissue, mouse skeletal muscle tissue, mouse heart tissue.
Molecular wt :
Predicted band size: 89 kDa
Subcellular location :
Cytoplasm, nucleus, endoplasmic reticulum
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IF-Cell: 1:250
;IHC-P: 1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ER30603_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ER30603_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ER30603_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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