Zfp352 | HUABIO ER2001-55 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Zfp352

catalog :

ER2001-55

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Non-transfected 293T cells lysate Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate Lane 3: Non-transfected 293F cells lysate Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

image 2 :

ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ER2001-55

Target name :

Zfp352

Species reactivity :

Mouse

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse Zfp352 aa 100-150.

Uniprot id :

A2AML7>SwissProt: A2AML7 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

GFP-tagged Zfp352 transfected 293T cells lysate, GFP-tagged Zfp352 transfected 293F cells lysate.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200

Advanced Validation :

Cell treatment (CT)

more info or order :

HUABIO product webpage