Phospho-p38 (T180) | HUABIO ER2001-51 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Phospho-p38 (T180)

catalog :

ER2001-51

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Phospho-p38 (T180) on different lysates with Rabbit anti-Phospho-p38 (T180) antibody (ER2001-51) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-51) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate, untreated with UV Lane 2: 293 cell lysate, treated with UV for 60 minutes

image 3 :

Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate, untreated with UV Lane 2: A431 cell lysate, treated with UV for 40 minutes

product information

SKU :

ER2001-51

Target name :

Phospho-p38 (T180)

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Synthetic phospho-peptide corresponding to residues surrounding Thr180 of human P38.

Uniprot id :

Q16539>SwissProt: Q16539 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

NIH/3T3 cell lysate, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes, Hela cell lysate treated with UV for 40 minutes.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER2001-51_4.jpg

Pic legend4 :

Western blot analysis of Phospho-p38 (T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes Lane 3: Hela cell lysate, treated with UV for 40 minutes

more info or order :

HUABIO product webpage