product summary
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company name :
HUABIO
product type :
antibody
product name :
TRPM4
catalog :
ER1902-81
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of TRPM4 on mouse colon tissue lysates with Rabbit anti-TRPM4 antibody (ER1902-81) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Exposure time: 1 minute 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of TRPM4 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ER1902-81
Target name :
TRPM4
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human TRPM4 aa 1-50 / 1,214.
Uniprot id :
Q8TD43>SwissProt: Q8TD43 Human;SwissProt: Q7TN37 Mouse;SwissProt: Q9ESQ5 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Mouse colon tissue lysates, MCF-7.
Molecular wt :
Predicted band size 134 kDa.
Subcellular location :
Cell membrane, endoplasmic reticulum, golgi apparatus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
;FC: 1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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