product summary
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company name :
HUABIO
product type :
antibody
product name :
USP29
catalog :
ER1902-76
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of USP29 on different lysates with Rabbit anti-USP29 antibody (ER1902-76) at 1/500 dilution.
Lane 1: Rat testis tissue lysate
Lane 2: Mouse testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Exposure time: 12 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-76) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of USP29 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: Rat testis tissue lysate
image 3 :
Flow cytometric analysis of USP29 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-76, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ER1902-76
Target name :
USP29
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human USP29 aa 665-922 / 922.
Uniprot id :
Q9HBJ7>SwissProt: Q9HBJ7 Human;SwissProt: Q9ES63 Mouse;SwissProt: D4AEI6 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Rat testis tissue lysate, mouse testis tissue lysate, THP-1 cell lysate, 293.
Molecular wt :
Predicted band size: 104 kDa
Subcellular location :
Cytoplasm, perinuclear region.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000
;FC: 1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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