product summary
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company name :
HUABIO
product type :
antibody
product name :
ATP8A1
catalog :
ER1902-61
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, rat
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of ATP8A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Rat brain tissue lysate
image 2 :
Flow cytometric analysis of ATP8A1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-61, 1/50) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
product information
SKU :
ER1902-61
Target name :
ATP8A1
Species reactivity :
Human,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human ATP8A1 aa 410-637 / 1,164.
Uniprot id :
Q9Y2Q0>SwissProt: Q9Y2Q0 Human;SwissProt: A0A8I5ZXX6 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
MCF-7 cell lysate, rat brain tissue lysate, MCF-7.
Molecular wt :
Predicted band size: 131 kDa
Subcellular location :
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane.
Concentration :
1 mg/ml.
Recommended dilutions :
WB: 1:500-1:1,000
;FC: 1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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