Claudin 18.1 | HUABIO ER1902-11 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Claudin 18.1

catalog :

ER1902-11

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Claudin 18.1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human stomach tissue lysate Lane 2: AGS cell lysate

image 2 :

Flow cytometric analysis of AGS cells labeling Claudin 18.1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER1902-11, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

image 3 :

Flow cytometric analysis of F9 cells labeling Claudin 18.1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER1902-11, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ER1902-11

Target name :

Claudin 18.1

Species reactivity :

Human,Mouse

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human Claudin18 aa 28-80.

Uniprot id :

P56856>SwissProt: P56856 Human;SwissProt: P56857 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human stomach tissue lysate, AGS cell lysate, AGS, F9.

Molecular wt :

Predicted band size 28 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:1,000 ;FC:1:500-1:1,000

more info or order :

HUABIO product webpage