TMEM163 | HUABIO ER1901-91 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TMEM163

catalog :

ER1901-91

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TMEM163 on different lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-91, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skin tissue lysate Lane 2: Human small intestine tissue lysate Lane 3: Mouse colon tissue lysate Predicted band size: 31 kDa Observed band size: 38 kDa

image 2 :

ICC staining of TMEM163 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-91, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-TMEM163 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER1901-91

Target name :

TMEM163

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TMEM163 aa 55-104 / 289.

Uniprot id :

Q8TC26>SwissProt: Q8TC26 Human;SwissProt: Q8C996 Mouse;SwissProt: A9CMA6 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human skin tissue lysate, human small intestine tissue lysate, human mouse colon tissue lysate, 293T, rat large intestine tissue, human prostate carcinoma tissue, F9.

Molecular wt :

Predicted band size 31 kDa

Subcellular location :

Early endosome membrane, synaptic vesicle membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500-1:2,000 ;IF-Cell:1:50-1:200 ;IHC-P:1:50-1:200 ;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1901-91_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using anti-TMEM163 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-91, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1901-91_5.jpg

Pic legend5 :

Flow cytometric analysis of TMEM163 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-91, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage