product summary
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company name :
HUABIO
product type :
antibody
product name :
CD133
catalog :
ER1901-63
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (ER1901-63) at 1/1,000 dilution.
Lane 1: NCCIT cell lysate (10 µg/Lane)
Lane 2: HT-29 cell lysate (10 µg/Lane)
Lane 3: Human kidney tissue lysate (20 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 120 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ER1901-63
Target name :
CD133
Species reactivity :
Human
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within C-terminal residues of human CD133.
Uniprot id :
O43490>SwissProt: O43490 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
NCCIT cell lysate, HT-29 cell lysate, Human kidney tissue lysate.
Molecular wt :
Predicted band size: 97 kDa
Subcellular location :
Cell membrane
Concentration :
1 mg/ml
Recommended dilutions :
WB: 1:500-1:2,000
;FC: 1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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