product summary
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company name :
HUABIO
product type :
antibody
product name :
Villin1
catalog :
ER1901-31
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO ER1901-31 image 1
Western blot analysis of Villin1 on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO ER1901-31 image 2
Flow cytometric analysis of Villin1 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
product information
SKU :
ER1901-31
Target name :
Villin1
Species reactivity :
Human,Mouse
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within human Villin aa 800-827.
Uniprot id :
P09327>SwissProt: P09327 Human;SwissProt: Q62468 Mouse
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Mouse colon tissue lysates, HCT116.
Molecular wt :
Predicted band size: 93 kDa
Subcellular location :
Cytoplasm, cytoskeleton, Cell projection.
Concentration :
1 mg/mL.
Recommended dilutions :
WB:1:500-1:1,000 ;FC:1:50-1:100
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!