MSH2 | HUABIO ER1803-86 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MSH2

catalog :

ER1803-86

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of MSH2 with anti-MSH2 antibody (ER1803-86) at 1:1,000 dilution. Lane 1: Wild-type SCC7 whole cell lysate. Lane 2: MSH2 knockout SCC7 whole cell lysate. ER1803-86 was shown to specifically react with MSH2 in wild-type SCC7 cells. No band was observed when MSH2 knockout samples were tested. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MSH2 antibody (ER1803-86, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MSH2 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ER1803-86

Target name :

MSH2

Species reactivity :

Human

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MSH2 aa 1-150.

Uniprot id :

P43246>SwissProt: P43246 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K562 cell lysates, Wild-type SCC7 whole cell lysates.

Molecular wt :

104 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000

Advanced Validation :

Knockout (KO)

more info or order :

HUABIO product webpage