product summary
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company name :
HUABIO
product type :
antibody
product name :
Estrogen Receptor alpha
catalog :
ER1803-83
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO ER1803-83 image 1
Western blot analysis of Estrogen Receptor alpha on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO ER1803-83 image 2
Flow cytometric analysis of Estrogen Receptor alpha was done on MCF-7 cells. The cells were fixed, permeabilized and stained with Estrogen Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
product information
SKU :
ER1803-83
Target name :
Estrogen Receptor alpha
Species reactivity :
Human,Mouse,Rat
Applications :
WB,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Estrogen Receptor alpha aa 1-120.
Uniprot id :
P03372>SwissProt: P03372 Human;SwissProt: P19785 Mouse;SwissProt: P06211 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
293T cell lysates, MCF-7.
Molecular wt :
66/54 kDa
Subcellular location :
Cytoplasm. Nucleus. Cell membrane. Golgi apparatus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000 ;FC: 1:50-1:100
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!