Nephrin | HUABIO ER1803-67 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Nephrin

catalog :

ER1803-67

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Nephrin on human kidney tissue lysates with Rabbit anti-Nephrin antibody (ER1803-67) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 135 kDa Observed band size: 200 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-67) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining Nephrin in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

ICC staining Nephrin in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

product information

SKU :

ER1803-67

Target name :

Nephrin

Species reactivity :

Human,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Nephrin aa 1,042-1,241 / 1,241.

Uniprot id :

O60500>SwissProt: O60500 Human;SwissProt: Q9R044 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human kidney tissue lysates, 293T, PANC-1, PC-12.

Molecular wt :

Predicted band size: 135 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/ml.

Recommended dilutions :

WB:1:1,000-1:2,000 ;IF-Cell:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-67_4.jpg

Pic legend4 :

ICC staining Nephrin in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

more info or order :

HUABIO product webpage