TMEM248 | HUABIO ER1803-48 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TMEM248

catalog :

ER1803-48

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: A-172 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 30 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of TMEM248 on different lysates with Rabbit anti-TMEM248 antibody (ER1803-48) at 1/5,000 dilution. Lane 1: K-562 cell lysate (no heat) Lane 2: HeLa cell lysate (hot lysis) Lane 3: PC-12 cell lysate Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 30 kDa Exposure time: 1 minute 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-48) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining Tmem248 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1803-48

Target name :

TMEM248

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human TMEM248 aa 265-314 / 314.

Uniprot id :

Q9NWD8>SwissProt: Q9NWD8 Human;SwissProt: Q3TBN1 Mouse;SwissProt: Q6AY76 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K-562 cell lysate, SK-Br-3 cell lysate, A-172 cell lysate, HeLa cell lysate, PC-12 cell lysate, HUVEC, SH-SY-5Y, SiHa.

Molecular wt :

Predicted band size: 35 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-48_4.jpg

Pic legend4 :

ICC staining Tmem248 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-48_5.jpg

Pic legend5 :

ICC staining Tmem248 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1803-48_6.jpg

Pic legend6 :

Flow cytometric analysis of SH-SY-5Y cells with Tmem248 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage