product summary
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company name :
HUABIO
product type :
antibody
product name :
APR3
catalog :
ER1803-44
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.
Lane 1: Anti-APR3 antibody (1/500).
Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.
image 2 :
ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ER1803-44
Target name :
APR3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within mouse APR3 aa 174-223 / 223.
Uniprot id :
Q6UW56>SwissProt: Q6UW56 Human;SwissProt: Q6PGD0 Mouse;SwissProt: B2RYU3 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
Human skin tissue lysates, A549, SH-SY-5Y, mouse kidney tissue, MCF-7.
Molecular wt :
Predicted band size: 25 kDa
Subcellular location :
Nucleus envelope, Cell membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-2,000;IF-Cell: 1:50-1:200;IHC-P: 1:500;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ER1803-44_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-APR3 antibody (ER1803-44) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-44) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ER1803-44_5.jpg
Pic legend5 :
Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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