ATP2A1 / SERCA1 | HUABIO ER1803-34 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATP2A1 / SERCA1

catalog :

ER1803-34

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ATP2A1 / SERCA1 on different lysates with Rabbit anti-ATP2A1 / SERCA1 antibody (ER1803-34) at 1/2,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Mouse skeletal muscle tissue lysate (no heat) Lane 3: Mouse cerebellum tissue lysate (no heat) (negative) Lane 4: Mouse heart tissue lysate (negative) Lane 5: Rat skeletal muscle tissue lysate Lane 6: Rat cerebellum tissue lysate (negative) Lane 7: Rat heart tissue lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 40 µg/Lane. Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ATP2A1 on different lysates using anti-ATP2A1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse skeletal muscle tissue Lane 2: Human skeletal muscle tissue

image 3 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1803-34

Target name :

ATP2A1 / SERCA1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetis peptide within mouse ATP2A1 aa 1-50 / 994.

Uniprot id :

O14983>SwissProt: O14983 Human;SwissProt: Q8R429 Mouse;SwissProt: Q64578 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, Human skeletal muscle tissue lysate, rat skeletal muscle tissue, human fetal skeletal muscle tissue, mouse skeletal muscle tissue.

Molecular wt :

Predicted band size: 110 kDa

Subcellular location :

Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-34_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-34_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-ATP2A1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1803-34_6.jpg

Pic legend6 :

Flow cytometric analysis of 293T cells with ATP2A1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage