CACNG1 | HUABIO ER1803-27 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CACNG1

catalog :

ER1803-27

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CACNG1 on human skeletal muscle lysate using anti-CACNG1 antibody at 1/1,000 dilution.

image 2 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1803-27

Target name :

CACNG1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within mouse CACNG1 aa 13-62 / 223.

Uniprot id :

Q06432>SwissProt: Q06432 Human;SwissProt: O70578 Mouse;SwissProt: P97707 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human skeletal muscle lysates, human fetal skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cell membrane, sarcolemma.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-27_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CACNG1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-27_5.jpg

Pic legend5 :

Immunocytochemistry analysis of SH-SY5Y cells labeling CACNG1 with Rabbit anti-CACNG1 antibody (ER1803-27) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CACNG1 antibody (ER1803-27) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1803-27_6.jpg

Pic legend6 :

Flow cytometric analysis of SH-SY5Y cells labeling CACNG1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1803-27, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage