Ryanodine receptor 2 / RYR-2 | HUABIO ER1803-22 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Ryanodine receptor 2 / RYR-2

catalog :

ER1803-22

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Dot blot analysis of anti-RYR2 immunization peptide on PVDF. 1ug, 2ug and 4ug peptides were given in this test. Anti-RYR2 antibody was diluted with 1/500.

image 2 :

Immunocytochemistry analysis of Siha cells labeling Ryanodine receptor 2 / RYR-2 with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (ER1803-22) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (ER1803-22) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of SH-SY-5Y cells labeling Ryanodine receptor 2 / RYR-2 with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (ER1803-22) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Ryanodine receptor 2 / RYR-2 antibody (ER1803-22) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ER1803-22

Target name :

Ryanodine receptor 2 / RYR-2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human RYR2 aa 1,471-1,520 / 4,967.

Uniprot id :

Q92736>SwissProt: Q92736 Human;SwissProt: E9Q401 Mouse;SwissProt: B0LPN4 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

SH-SY-5Y, Siha, human fetal skeletal muscle tissue, mouse brain tissue, mouse cerebellum tissue, rat skeletal muscle tissue.

Molecular wt :

565 kDa

Subcellular location :

Membrane. Sarcoplasmic reticulum.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-22_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-22_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RYR2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1803-22_6.png

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-RYR2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1803-22_7.jpg

Pic legend7 :

Flow cytometric analysis of 293T cells with RYR2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage