GPX4 | HUABIO ER1803-15 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GPX4

catalog :

ER1803-15

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ER1803-15) at 1/1,000 dilution. Lane 1: Rat testis tissue lysate (20 µg/Lane) Lane 2: Mouse testis tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-15) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining GPX4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining GPX4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1803-15

Target name :

GPX4

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human GPX4 aa 1-100 / 197.

Uniprot id :

P36969>SwissProt: P36969 Human;SwissProt: O70325 Mouse;SwissProt: P36970 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Rat testis tissue lysate, HepG2, LOVO, MCF-7, mouse testis tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm. Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:50 -1:600 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-15_4.jpg

Pic legend4 :

ICC staining GPX4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-15_5.png

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- GPX4 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1803-15_6.jpg

Pic legend6 :

Flow cytometric analysis of HepG2 cells with GPX4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage