product summary
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company name :
HUABIO
product type :
antibody
product name :
NDUFS3
catalog :
ER1803-14
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO ER1803-14 image 1
Western blot analysis of NDUFS3 on different lysates with Rabbit anti-NDUFS3 antibody (ER1803-14) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-NDUFS3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-14) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO ER1803-14 image 2
Western blot analysis of NDUFS3 on different cell lysates using anti-NDUFS3 antibody at 1/500 dilution. Positive control: Lane 1: LOVO Lane 2: MCF-7
image 3 :
HUABIO ER1803-14 image 3
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
product information
SKU :
ER1803-14
Target name :
NDUFS3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human NDUFS3 aa 7-239 / 264.
Uniprot id :
O75489>SwissProt: O75489 Human;SwissProt: Q9DCT2 Mouse;SwissProt: D3ZG43 Rat
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
LOVO, MCF-7, rat epididymis tissue, human liver tissue, mouse brain tissue.
Molecular wt :
Predicted band size: 30 kDa
Subcellular location :
Mitochondrion inner membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:1,000 ;IHC-P: 1:50-1:2,000 ;FC: 1:50-1:100
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ER1803-14_4.png
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ER1803-14_5.png
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NDUFS3 antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ER1803-14_6.jpg
Pic legend6 :
Flow cytometric analysis of MCF-7 cells with NDUFS3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!