USP13 | HUABIO ER1803-09 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

USP13

catalog :

ER1803-09

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of USP13 on different lysates with Rabbit anti-USP13 antibody (ER1803-09) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-USP13 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-09) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Daudi Lane 3: Mouse testis Lane 4: Rat brain

image 3 :

ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1803-09

Target name :

USP13

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,FC,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human USP13 aa 570-776 / 863.

Uniprot id :

Q92995>SwissProt: Q92995 Human;SwissProt: Q5BKP2 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.

Molecular wt :

97 kDa

Subcellular location :

Nucleus. Cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100 ;IHC-P: 1:100-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1803-09_4.jpg

Pic legend4 :

ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1803-09_5.jpg

Pic legend5 :

Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage