Bcl-2 | HUABIO ER1802-97 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Bcl-2

catalog :

ER1802-97

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Bcl2 on different lysates using anti-Bcl2 antibody at 1/200 dilution. Positive control: Lane 1: HL-60 Lane 2: Mouse colon

image 2 :

ICC staining Bcl2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Bcl2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1802-97

Target name :

Bcl-2

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human BCL-2 20-100 aa.

Uniprot id :

P10415>SwissProt: P10415 Human;SwissProt: P10417 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.

Molecular wt :

22/26 kDa

Subcellular location :

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:200-1:500 ;IF-Cell: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-97_4.jpg

Pic legend4 :

ICC staining Bcl2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-97_5.jpg

Pic legend5 :

Flow cytometric analysis of MCF-7 cells with Bcl2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage