Fibrillarin | HUABIO ER1802-81 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Fibrillarin

catalog :

ER1802-81

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: NIH-3T3 cell lysate

image 2 :

Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-81) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ER1802-81

Target name :

Fibrillarin

Species reactivity :

Human,Mouse

Applications :

WB,FC,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Fibrillarin aa 272-321 / 321.

Uniprot id :

P22087>SwissProt: P22087 Human;SwissProt: P35550 Mouse

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Hela cell lysate, HepG2 cell lysate, 293T cell lysate, NIH-3T3 cell lysate, HL-60, human tonsil tissue, mouse liver tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;FC: 1:50-1:100 ;IHC-P: 1:200-1:1,000 ;IF-Tissue: 1:50-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-81_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-81) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-81_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Fibrillarin with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-81, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-81_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling Fibrillarin with Rabbit anti-Fibrillarin antibody (ER1802-81) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-81, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage