product summary
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company name :
HUABIO
product type :
antibody
product name :
BubR1
catalog :
ER1802-76
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of BubR1 on SH-SY5Y cell lysates with Rabbit anti-BubR1 antibody (ER1802-76) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-76) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining BubR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
ICC staining BubR1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ER1802-76
Target name :
BubR1
Species reactivity :
Human
Applications :
WB,IF-Cell,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human BubR1 aa 310-620.
Uniprot id :
O60566>SwissProt: O60566 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
SH-SY5Y cell lysates, Hela, SH-SY-5Y, SiHa, human spleen tissue, HL-60.
Molecular wt :
Predicted band size: 120 kDa
Subcellular location :
Nucleus, Cytoplasm, Cytoskeleton.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:5,000
;IF-Cell: 1:50-1:200
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ER1802-76_4.jpg
Pic legend4 :
ICC staining BubR1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ER1802-76_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BubR1 antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ER1802-76_6.jpg
Pic legend6 :
Flow cytometric analysis of HL-60 cells with BubR1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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