PKM2 | HUABIO ER1802-70 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PKM2

catalog :

ER1802-70

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1802-70) at 1:500 dilution. Lane 1: Wild-type MDA-MB-231 whole cell lysate. Lane 2: PKM knockout MDA-MB-231 whole cell lysate. ER1802-70 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1802-70, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 2 :

Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (ER1802-70) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: PC-3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-70) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 3 :

ICC staining PKM2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1802-70

Target name :

PKM2

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human PKM2 aa 370-450.

Uniprot id :

P14618>SwissProt: P14618 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Wild-type MDA-MB-231 whole cell lysate, SiHa cell lysate, A549 cell lysate, PC-3 cell lysate, A549, F9, PC-3M, human tonsil tissue, human thyroid carcinoma tissue, human colon cancer tissue, human breast cancer tissue.

Molecular wt :

Predicted band size: 58 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:50-1:400

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_4.jpg

Pic legend4 :

ICC staining PKM2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_5.jpg

Pic legend5 :

ICC staining PKM2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKM2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-PKM2 antibody (ER1802-70) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-70) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1802-70_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage