APE1 | HUABIO ER1802-49 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

APE1

catalog :

ER1802-49

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of APE1 on different lysates with Rabbit anti-APE1 antibody (ER1802-49) at 1/5,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD APE1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-49) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution. Positive control: Lane 1: HL-60 Lane 2: Human skin tissue Lane 3: MCF-7 Lane 4: Mouse placenta tissue

image 3 :

ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1802-49

Target name :

APE1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human APE1 aa 2-270.

Uniprot id :

P27695>SwissProt: P27695 Human;SwissProt: P28352 Mouse;SwissProt: P43138 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HL-60, human skin tissue lysate, mouse placenta tissue lysate, MCF-7, HepG2, LOVO, PC-3M, human liver tissue, human colon tissue, human breast cancer tissue, A549.

Molecular wt :

Predicted band size: 36 kDa

Subcellular location :

Nucleus, Cytoplasm, Endoplasmic reticulum.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:500-1:1,000 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_4.jpg

Pic legend4 :

ICC staining APE1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_5.jpg

Pic legend5 :

ICC staining APE1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-APE1 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-APE1 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1802-49_9.jpg

Pic legend9 :

Flow cytometric analysis of A549 cells with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage