GOT1 | HUABIO ER1802-48 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GOT1

catalog :

ER1802-48

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GOT1 on different lysates with Rabbit anti-GOT1 antibody (ER1802-48) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-GOT1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-48) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin.

product information

SKU :

ER1802-48

Target name :

GOT1

Species reactivity :

Human

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within N-terminal Human GOT1.

Uniprot id :

P17174>SwissProt: P17174 Human

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

K562, human liver tissue, human colon tissue, human heart tissue.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-48_4.png

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-48_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-48_6.jpg

Pic legend6 :

Flow cytometric analysis of HepG2 cells with GOT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage