Cytokeratin 8 | HUABIO ER1802-43 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Cytokeratin 8

catalog :

ER1802-43

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Cytokeratin 8 on different lysates using anti-Cytokeratin 8 antibody at 1/500 dilution. Positive control: Lane 1: Human small intestine Lane 2: A431

image 2 :

ICC staining Cytokeratin 8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Cytokeratin 8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1802-43

Target name :

Cytokeratin 8

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Cytokeratin 8.

Uniprot id :

P05787>SwissProt: P05787 Human;SwissProt: P11679 Mouse;SwissProt: Q10758 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

Human small intestine tissue lysate, A431, A549, LOVO, MCF-7, rat epididymis tissue, human lung cancer tissue, human prostate tissue, mouse liver tissue.

Molecular wt :

Predicted band size: 54 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_4.jpg

Pic legend4 :

ICC staining Cytokeratin 8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 8 antibody. Counter stained with hematoxylin.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1802-43_9.jpg

Pic legend9 :

Flow cytometric analysis of MCF-7 cells with Cytokeratin 8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage