APC | HUABIO ER1802-39 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

APC

catalog :

ER1802-39

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

ICC staining APC in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 2 :

ICC staining APC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining APC in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ER1802-39

Target name :

APC

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within C-terminal human Ifna1.

Uniprot id :

P25054>SwissProt: P25054 Human;SwissProt: Q61315 Mouse;SwissProt: P70478 Rat

Host :

Rabbit

Isotype :

IgG

Size :

100μl

List Price :

330 USD

Storage Buffer :

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Immunogen affinity purified.

Product type :

Rabbit polyclonal Antibody

Positive control :

HUVEC, SH-SY-5Y, SiHa, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 312 kDa

Subcellular location :

Cell junction. Cytoplasm. Cytoskeleton. Membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:1,000 ;FC: 1:50-1:100 ;WB: 1:500 ;IF-Tissue: 1:200 ;IHC-Fr: 1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-APC antibody (ER1802-39) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-39) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-APC antibody (ER1802-39) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-39) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-APC antibody (ER1802-39) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1802-39) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_7.jpg

Pic legend7 :

Flow cytometric analysis of SH-SY-5Y cells with APC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_8.jpg

Pic legend8 :

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling APC with Rabbit anti-APC antibody (ER1802-39) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-39, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ER1802-39_9.jpg

Pic legend9 :

Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-APC antibody (ER1802-39) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ER1802-39, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

more info or order :

HUABIO product webpage