product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
KDM5B
catalog :
ER1802-21
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of KDM5B on different lysates with Rabbit anti-KDM5B antibody (ER1802-21) at 1/1,000 dilution.
Lane 1: BT-20 cell lysate (16 µg/Lane)
Lane 2: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 3: SGC7901 cell lysate (20 µg/Lane)
Lane 4: AGS cell lysate (20 µg/Lane)
Lane 5: A549 cell lysate (20 µg/Lane)
Lane 6: NCI-H441 cell lysate (20 µg/Lane)
Predicted band size: 176 kDa
Observed band size: 150 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-21) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ER1802-21
Target name :
KDM5B
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human KDM5B aa 960-1,009 / 1,544.
Uniprot id :
Q9UGL1>SwissProt: Q9UGL1 Human
Host :
Rabbit
Isotype :
IgG
Size :
100μl
List Price :
330 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Rabbit polyclonal Antibody
Positive control :
BT-20 cell lysate, MDA-MB-468 cell lysate, SGC7901 cell lysate, AGS cell lysate, A549 cell lysate, NCI-H441 cell lysate.
Molecular wt :
Predicted band size: 176 kDa
Subcellular location :
Nucleus.
Concentration :
2 mg/mL.
Recommended dilutions :
WB: 1:1,000
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
related products
browse more products
questions and comments